Single-step purification and structural characterization of human interleukin-6 produced in Esherichia coli From a T7 RNA polymerase expression vector
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Affinity Purification and Characterization of Recombinant Bacillus sphaericus Phenylalanine Dehydrogenase Produced by pET Expression Vector System
Cloning and expression of the L-phenylalanine dehydrogenase gene, from B. sphaericus in E. coli were done. The gene was cloned in the vector pET16b and transformed into E. coli BL21 (DE3). The functional form of the L-phenylalanine dehydrogenase enzyme was purified by affinity purification techniques, taking advantage of the ability of this enzyme to bind to the nucleotide site affinity dye, Re...
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There is a permanent and increasing interest in large amounts of RNAs synthesized in vitro by bacteriophage T7 RNA polymerase. Especially for physical studies, mg quantities of RNA are required and have in fact been synthesized (1, 2). In order to encounter the resulting demand for T7 polymerase, we have developed a simple purification scheme for mis enzyme, using the expression system of Studi...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1991
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1991.tb16048.x